NEIST-SOPHISTICATED ANALYTICAL INSTRUMENT FACILITY (NEIST-SAIF)

Sophisticated Analytical Instruments Facility

Name of the equipment/facility: Confocal Laser Scanning Microscopy(CLSM)
Make: Leica Microsystems, Germany
Model: Leica TCS SP8
Specification:
- Bright field, Fluorescence and differential interface contrast (DIC) illumination with facility for confocal scan head attachment - Automatic (motorized) beam path selection for visual and confocal imaging - Detectors are capable of working in Intensity and Spectral mode Imaging. Should be capable of simultaneous detection and separation of minimum 5 fluorophores. The detection unit is a combination of 2 PMT & 3 GaAsP/HyD. - The scan head should be able to perform fast dynamic live cell time lapse imaging with ROI capability with a high speed of 100-300 fps or better @512X32 resolution OR scan speed should be 7-10fps or better @512X512 resolution - Lasers: Pre aligned Solid State/ Gas State laser module with laser lines of a) Multi-line Ar laser with 458/488/514nm. b) DPSS 561 nm c) HeNe 633 nm d) Blue Diode Laser 405/408nm. Microsocpe is equipped with either peizo stage or bidirectional galvo stage for fastest possible Z sections, with travel range of at least 100 microns or better for fast imaging in Confocal and SR mode.
User Instructions:
- User has to bring their samples personally or send the mounted samples on permanent slides suitable to fit in inverted microscope of CLSM - User has to provide complete sample details with type of tagging or stain, suitable wavelength, need of co-localization, live imaging etc. depending on the application and accordingly the samples will be charged - Users are encouraged to be present during the analysis of their samples. Users who are interested in specialised experiments are advised to contact Central Instrumentation facility, Bhatnagar Building of CSIR-NEIST, Jorhat-785 006 for an appointment. All users are required to acknowledge the use of the facility when the results are published or presented in a symposium / conference. It will be highly appreciated if users send a Xerox copy of such publication/report to the Central Instrumentation facility, Bhatnagar Building of CSIR-NEIST, Jorhat-785 006. - User should bring their own DVD/ CD for data transfer. No USB or pen drive allowed. - All samples should accompany material safety data sheets (MSDS).
Applications:
Confocal Laser Scanning Microscope is one of the most vital high end equipment to perform wide range of cell biological applications such as co-localization analysis of fluorescently labelled proteins, live 3D imaging, FRET analysis, sub-cellular localization and expression of proteins of plants, bacteria and animal models, in vivo protein-protein interaction assays, detection and analysis of immunolabelled proteins, live video recording of movement of cellular organelles and movement of fluorescently tagged proteins in the cell etc.
Contact
Dr.ChannaChikkaputtaiah, Senior Scientist, CSIR-NEIST Jorhat-785006. E-mail:channakeshav@neist.res.in
Charges (Excluding Taxes)

Charges (Excluding Taxes):

Particulars

Industry

University

National Labs

Per sample/ per hour

Instrumentation

5475

4350

4350

Per hour

Operator assisted

5890

4670

4670

Per hour

Expertise assisted

6310

5000

5000

Per hour